Improving Effect of Ethyl Acetate Fraction of Tanacetum Parthenium Against Brain Oxidative Damage in Pentylenetetrazole-Induced Seizure Model In Mice

Aims: Oxidative stress plays an important role in pathogenesis of seizure. In this study the effect of ethyl acetate fraction of Tanacetum parthenium against brain oxidative damage in pentylenetetrazole-induced (PTZ) seizure model in mice was investigated. Materials and methods: In this experimental study, mice were distributed into 6 groups: 1) Control, 2) PTZ, 3-6) Fraction+PTZ. The animals of 4 recent groups, besides PTZ received 25, 50,100 and 200 mg/kg of fraction. PTZ (100 mg/kg) and fraction (30 min before PTZ) were administered interapretoneally for 3 weeks. Delay in onset of Minimal Clonic Seizur (MCS), Generalized Tonic-Clonic Seizure (GTCS) and the level of oxidative stress indexes in cortical and hippocampal tissues were measured.  Results: Pretreatment with fraction resulted in postponing the onset seizures in Fraction+PTZ groups compared to PTZ group (P<0.05, P<0.01 and P<0.001). In addition, all doses of fraction reduced malondialdehyde (MDA) concentration and increased the level of total thiol and superoxide dismutase (SOD) and catalase (CAT) activity in brain tissues of Fraction+PTZ groups with respect to PTZ group (P<0.05, P<0.01 and P<0.001).   Conclusion: Ethyl acetate fraction of Tanacetum parthenium attenuated PTZ- stimulated seizures through improving brain tissue oxidative stress.